Aliases:	GLS; Glutaminase; GLS1; K-Glutaminase; KIAA0838; GAC; GAM; KGA; Glutaminase Kidney Isoform, Mitochondrial; L-Glutamine Amidohydrolase; Glutaminase C; EC 3.5.1.2; Glutaminase, Phosphate; Activated; CASGID; EIEE71; AAD20; DEE71; GDPAG
Background:	UniProt Entry: O94925;NCBI Gene Entry: 2744
Application Information	Molecular Weight: Predicted, 73 kDa, observed, 55-65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1720 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Glutaminase
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Glutaminase expression in HepG2 cells using Glutaminase antibody 1:2,000. Green, isotype control; red, Glutaminase.

Immunocytochemical staining of HepG2 cells with Glutaminase antibody 1:1,000. Nuclei were stained blue with DAPI; Glutaminase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Glutaminase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Glutaminase, caseinolytic mitochondrial matrix peptidase chaperone subunit X.

Western blotting analysis using anti-Glutaminase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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