Aliases:	Histone Deacetylase 3; RPD3-2; HD3 KDAC3; RPD3; Protein Deacetylase HDAC3; Protein Deacylase HDAC3; EC 3.5.1.98; SMAP45; EC 3.5.1.-
Background:	UniProt Entry: O15379;NCBI Gene Entry: 8841
Application Information	Molecular Weight: Predicted, 49 kDa, observed, 49 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB860 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HDAC3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of HDAC3 expression in HepG2 cells using HDAC3 antibody 1:1,000. Nuclei were stained blue with DAPI; HDAC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-HDAC3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-HDAC3 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

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