Aliases:	Histone Deacetylase 4; HD4; KIAA0288; HDAC-A; HA6116; HDAC-4; HDACA; EC 3.5.1.98; BDMR; Brachydactyly-Mental Retardation Syndrome; Histone Deacetylase A; NEDCHID; NEDCHF; AHO3
Background:	UniProt Entry: P56524;NCBI Gene Entry: 9759
Application Information	Molecular Weight: Predicted, 120 kDa, observed, 140 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB865 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HDAC4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of HDAC4 expression in HepG2 cells using HDAC4 antibody 1:2,000. Green, isotype control; red, HDAC4.

Immunocytochemical staining of HepG2 cells with HDAC4 antibody 1:1,000. Nuclei were stained blue with DAPI; HDAC4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-HDAC4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-HDAC4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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