Aliases:	HIF1AN; Hypoxia Inducible Factor 1 Subunit Alpha Inhibitor; FIH1; Hypoxia-Inducible Factor Asparagine Hydroxylase; Hypoxia-Inducible Factor 1-Alpha Inhibitor; Peptide-Aspartate Beta-Dioxygenase; Factor Inhibiting HIF-1; FLJ20615; FLJ22027; FIH-1; Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor; Factor Inhibiting HIF1; DKFZp762F1811; DKFZP762F1811; EC 1.14.11.30; EC 1.14.11.N4
Background:	UniProt Entry: Q9NWT6;NCBI Gene Entry: 55662
Application Information	Molecular Weight: Predicted, 40 kDa, observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6385 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HIF1AN
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of HIF1AN expression in HeLa cells using HIF1AN antibody 1:2,000. GrHIF1ANotype control; red, HIF1AN.

Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody 1:1,000. Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-HIF1AN antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-HIF1AN antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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