Aliases:	HSF1; Heat Shock Transcription Factor 1; HSTF1; Heat Shock Factor Protein 1; HSTF; HSF
Background:	UniProt Entry: Q00613;NCBI Gene Entry: 3297
Application Information	Molecular Weight: Predicted, 57 kDa, observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB5405 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM)
Immunogen	A synthesized peptide derived from human HSF1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-HSF1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using FeQTM ECL Substrate Kit .

Flow cytometric analysis of HSF1 expression in HepG2 cells using anti-HSF1 antibody 1:2,000. Green, isotype control; red,HSF1.

Immunocytochemical staining of HepG2 cells with anti-HSF1 antibody 1:1,000. Nuclei were stained blue with DAPI; HSF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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