Aliases:	HSPB8; Heat Shock Protein Family B (Small) Member 8; E2IG1; HSP22; CMT2L; H11; Small Stress Protein-Like Protein HSP22; Heat Shock 27kDa Protein 8; Heat Shock 22kDa Protein 8; Heat Shock Protein Beta-8; E2-Induced Gene 1 Protein; Alpha-Crystallin C Chain; Protein Kinase H11; HSPB8-N1; HSPB8-N2; DHMN2; HMN2A; CRYAC; HspB8; HMN2
Background:	UniProt Entry: Q9UJY1;NCBI Gene Entry: 26353
Application Information	Molecular Weight: Predicted, 22 kDa, observed, 22 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1980 Spcies Reaceivity: Human, Mouse Applicseions Tsted: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HSPB8/HSP22
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Hsp22 expression in HepG2 cells using Hsp22 antibody 1:2,000. Green, isotype control; red, Hsp22.

Immunocytochemical staining of HepG2 cells with Hsp22 antibody 1:1,000. Nuclei were stained blue with DAPI; Hsp22 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Hsp22 antibody 1:2,500 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Hsp22 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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