Aliases:	IFIH1; Interferon Induced With Helicase C Domain 1; MDA-5; MDA5; Helicard; IDDM19; Interferon-Induced Helicase C Domain-Containing Protein 1; Clinically Amyopathic Dermatomyositis Autoantigen 140 KDa; Melanoma Differentiation-Associated Protein 5; Melanoma Differentiation-Associated Gene 5; RNA Helicase-DEAD Box Protein 116; Murabutide Down-Regulated Protein; Helicase With 2 CARD Domains; RIG-I-Like Receptor; CADM-140 Autoantigen; RLR-2; Hlcd; Interferon-Induced With Helicase C Domain Protein 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide; EC 3.6.4.13; SGMRT1; IMD95; RH116; AGS7; HLCD
Background:	UniProt Entry: Q9BYX4;NCBI Gene Entry: 64135
Application Information	Molecular Weight: Predicted, 117 kDa, observed, 140 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6085 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human MDA5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-IFIH1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using FeQTM ECL Substrate Kit .

Western blotting analysis using anti-IFIH1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:1,000. Nuclei were stained blue with DAPI; IFIH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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