Aliases:	Integrin; Alpha 5 (Fibronectin Receptor,Alpha Polypeptide); VLA5A FNRA; CD49e; Fibronectin Receptor Subunit Alpha; Fibronectin Receptor Alpha Subunit; Integrin Alpha-F; Integrin Alpha-5; VLA-5; Very Late Activation Protein 5; Alpha Subunit; CD49 Antigen-Like Family Member E; CD49e Antigen
Background:	UniProt Entry: P08648;NCBI Gene Entry: 3678
Application Information	Molecular Weight: Predicted, 115 kDa, observed, 150 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1050 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Integrin alpha 5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Integrin alpha 5 expression in C2C12 cells using Integrin alpha 5 antibody 1:2,000. Green, isotype control; red, Integrin alpha 5.

Immunocytochemical staining of C2C12 cells with Integrin alpha 5 antibody 1:1,000. Nuclei were stained blue with DAPI; Integrin alpha 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Integrin alpha 5 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-Integrin alpha 5 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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