Aliases:	KDM1B; Lysine Demethylase 1B; LSD2; AOF1; Flavin-Containing Amine Oxidase Domain-Containing Protein 1; Lysine-Specific Histone Demethylase 1B; Lysine-Specific Histone Demethylase; Lysine (K)-Specific Demethylase 1B; DJ298J15.2; BA204B7.3; FLJ34109; FLJ33898; FLJ43328; C6orf193; Amine Oxidase (Flavin Containing) Domain 1; Chromosome 6 Open Reading Frame 193; Amine Oxidase, Flavin Containing 1; EC 1.14.99.66; C6ORF193
Background:	UniProt Entry: Q8NB78;NCBI Gene Entry: 221656
Application Information	Molecular Weight: Predicted, 92 kDa, observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6265 Spncies Reactivity: Human, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human LSD2 / AOF1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Lysine demethylase 1B expression in HepG2 cells using Lysine demethylase 1B antibody 1:2,000. Green, isotype control; red, Lysine demethylase 1B.

Immunocytochemical staining of HepG2 cells with anti-Lysine demethylase 1B antibody 1:1,000. Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Lysine demethylase 1B antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Lysine demethylase 1B antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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