Aliases:	LAMC1; Laminin Subunit Gamma 1; Laminin B2 Chain; LAMB2; Laminin, Gamma 1 (Formerly LAMB2); Laminin-10 Subunit Gamma; Laminin-11 Subunit Gamma; Laminin Subunit Gamma-1; S-Laminin Subunit Gamma; Laminin-2 Subunit Gamma; Laminin-3 Subunit Gamma; Laminin-4 Subunit Gamma; Laminin-6 Subunit Gamma; Laminin-7 Subunit Gamma; Laminin-8 Subunit Gamma; Laminin-9 Subunit Gamma; S-LAM Gamma; Laminin-1 Subunit Gamma
Background:	UniProt Entry: P11047;NCBI Gene Entry: 3915
Application Information	Molecular Weight: Predicted, 178 kDa, observed, 220-250 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB5925 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM)
Immunogen	A synthesized peptide derived from human Laminin gamma 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-laminin subunit gamma 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:1,000. Nuclei were stained blue with DAPI; Laminin subunit gamma 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Flow cytometric analysis of laminin subunit gamma 1 expression in HAP-1 cells using anti-laminin subunit gamma 1 antibody 1:2,000. Green, isotype control; red, laminin subunit gamma 1.

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