Aliases:	MCAM; Melanoma Cell Adhesion Molecule; MUC18; METCAM; HEMCAM; CD146; S-Endo 1 Endothelial-Associated Antigen; Melanoma-Associated Antigen MUC18; Cell Surface Glycoprotein MUC18; Cell Surface Glycoprotein P1H12; Melanoma-Associated Antigen A32; Gicerin; MelCAM; Melanoma Adhesion Molecule; CD146 Antigen; MELCAM
Background:	UniProt Entry: P43121;NCBI Gene Entry: 4162
Application Information	Molecular Weight: Predicted, 72 kDa, observed, 100 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2705 Spncies Reactivity: Human, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CD146
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of CD146 expression in HepG2 cells using CD146 antibody 1:2,000. Green, isotype control; red, CD146.

Immunocytochemical staining of HepG2 cells with CD146 antibody 1:1,000. Nuclei were stained blue with DAPI; CD146 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CD146 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-CD146 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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