Aliases:	Minichromosome Maintenance Complex Component 7; CDC47; PPP1R104; MCM2; Protein Phosphatase 1, Regulatory Subunit 104; DNA Replication Licensing Factor MCM7; CDC47 Homolog; P1.1-MCM3; MCM7 Minichromosome Maintenance Deficient 7 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 7; Minichromosome Maintenance Deficient 7; Homolog Of S. Cerevisiae Cdc47; EC 3.6.4.12; P1CDC47; PNAS146; P85MCM
Background:	UniProt Entry: P33993;NCBI Gene Entry: 4176
Application Information	Molecular Weight: Predicted, 81 kDa, observed, 81 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB925 Spncies Reactivity: Human, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human MCM7
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of MCM7 expression in HepG2 cells using MCM7 antibody 1:2,000. Green, isotype control; red, MCM7.

Immunocytochemical staining of HepG2 cells with MCM7 antibody 1:1,000. Nuclei were stained blue with DAPI; MCM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-MCM7 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-MCM7 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

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