Aliases:	Splicing Factor Proline And Glutamine Rich; PSF; PPP1R140; Polypyrimidine Tract Binding Protein Associated; Protein Phosphatase 1, Regulatory Subunit 140; DNA-Binding P52/P100 Complex, 100 KDa Subunit; Splicing Factor, Proline- And Glutamine-Rich; Splicing Factor Proline/Glutamine-Rich; 100 KDa DNA-Pairing Protein; Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract-Binding Protein-Associated); Splicing Factor Proline/Glutamine Rich (Polypyrimidine Tract Binding Protein Associated); Polypyrimidine Tract-Binding Protein-Associated Splicing Factor; Polypyrimidine Tract-Binding Protein-Associated-Splicing Factor; Epididymis Secretory Sperm Binding Protein; PTB-Associated Splicing Factor; PTB-Associated-Splicing Factor; HPOMp100; POMP100
Background:	UniProt Entry: P23246;NCBI Gene Entry: 6421
Application Information	Molecular Weight: Predicted, 76 kDa, observed, 100 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1220 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SFPQ
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of SFPQ expression in HepG2 cells using anti-SFPQ antibody 1:2,000. Green, isotype control; red, SFPQ.

Immunocytochemical staining of HepG2 cells with anti-SFPQ antibody 1:1,000. Nuclei were stained blue with DAPI; SFPQ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-SFPQ antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-SFPQ antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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