Aliases:	MMUT; Methylmalonyl-CoA Mutase; MCM; MUT; Methylmalonyl-CoA Mutase, Mitochondrial; Methylmalonyl Coenzyme A Mutase; Methylmalonyl-CoA Isomerase; EC 5.4.99.2; Methylmalonyl-CoA Mutase Variant C.613_615delGAA; Methylmalonyl-CoA Mutase Variant C.1495G>A; Methylmalonyl-CoA Mutase Variant C.2011A>G; Methylmalonyl-CoA Mutase Variant C.2150G>T; Methylmalonyl-CoA Mutase Variant C.322C>T; Methylmalonyl-CoA Mutase Variant C.636G>A; Methylmalonyl-CoA Mutase Variant C.643G>A; Methylmalonyl-CoA Mutase C.*192delA; Methylmalonyl-CoA Mutase C.*51C>G; Mutant Methylmalonyl CoA Mutase
Background:	UniProt Entry: P22033;NCBI Gene Entry: 4594
Application Information	Molecular Weight: Predicted, 83 kDa, observed, 78 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4555 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human MMUT
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Methylmalonyl-CoA mutase expression in HepG2 cells using Methylmalonyl-CoA mutase antibody 1:2,000. Green, isotype control; red, Methylmalonyl-CoA mutase.

Immunocytochemical staining of HepG2 cells with Methylmalonyl-CoA mutase antibody 1:1,000. Nuclei were stained blue with DAPI; Methylmalonyl-CoA mutase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very Low. Scale bar: 20 μm.

Western blotting analysis using anti-Methylmalonyl-CoA mutase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Methylmalonyl-CoA mutase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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