Aliases:	Nemo Like Kinase; Serine/Threonine-Protein Kinase NLK; Nemo-Like Kinase; EC 2.7.11.24; Protein LAK1; EC 2.7.11; LAK1
Background:	UniProt Entry: Q9UBE8;NCBI Gene Entry: 51701
Application Information	Molecular Weight: Predicted, 58 kDa, observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB585 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NLK
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Nemo like kinase expression in HeLa cells using Nemo like kinase antibody 1:2,000. Green, isotype control; red, Nemo like kinase.

Immunocytochemical staining of HeLa cells with anti-Nemo like kinase antibody 1:1,000. Nuclei were stained blue with DAPI; Nemo like kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Nemo like kinase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Nemo like kinase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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