Aliases:	NAD(P) Dependent Steroid Dehydrogenase-Like; SDR31E1; XAP104; H105e3; Sterol-4-Alpha-Carboxylate 3-Dehydrogenase, Decarboxylating; Short Chain Dehydrogenase/Reductase Family 31E, Member 1; Protein H105e3; EC 1.1.1.170; NAD(P) Dependent Steroid Dehydrogenase-Like Protein Transcript; Epididymis Secretory Sperm Binding Protein; H105E3
Background:	UniProt Entry: Q15738;NCBI Gene Entry: 50814
Application Information	Molecular Weight: Predicted, 42 kDa, observed, 38 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB510 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NSDHL
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NSDHL expression in HT-1080 cells using anti-NSDHL antibody 1:2,000. Green, isotype control; red, NSDHL.

Immunocytochemical staining of HT-1080 cells with anti-NSDHL antibody 1:1,000. Nuclei were stained blue with DAPI; NSDHL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NSDHL antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NSDHL antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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