Aliases:	Poly(A) Binding Protein Nuclear 1; PAB2 ; PABP2; Polyadenylate-Binding Protein 2; PABP-2; PABII; OPMD; Polyadenylate-Binding Nuclear Protein 1; Poly(A)-Binding Protein, Nuclear 1; Poly(A) Binding Protein, Nuclear 1; Nuclear Poly(A)-Binding Protein 1; Poly(A) Binding Protein II; Poly(A)-Binding Protein II; Poly(A) Binding Protein 2; Poly(A)-Binding Protein 2
Background:	UniProt Entry: Q86U42;NCBI Gene Entry: 8106
Application Information	Molecular Weight: Predicted, 33 kDa, observed, 49 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB365 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PABPN1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of PABPN1 expression in HepG2 cells using PABPN1 antibody 1:2,000. Green, isotype control; red, PABPN1.

Immunocytochemical staining of Hela cells with anti-PABPN1 antibody 1:1,000. Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-PABPN1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-PABPN1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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