Aliases:	Pyruvate Dehydrogenase Kinase 1; [Pyruvate Dehydrogenase (Acetyl-Transferring)] Kinase Isozyme 1, Mitochondrial; Pyruvate Dehydrogenase Kinase, Isoenzyme 1; PDH Kinase 1; EC 2.7.11.2; Pyruvate Dehydrogenase (Acetyl-Transferring) Kinase Isozyme 1, Mitochondrial; Mitochondrial Pyruvate Dehydrogenase, Lipoamide, Kinase Isoenzyme 1; Pyruvate Dehydrogenase Kinase, Isozyme 1; Pyruvate Dehydrogenase Kinase Isoform 1; EC 2.7.11; PDHK1
Background:	UniProt Entry: Q15118;NCBI Gene Entry: 5163
Application Information	Molecular Weight: Predicted, 49 kDa, observed, 49 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1190 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM)
Immunogen	A synthesized peptide derived from human PDK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Pyruvate dehydrogenase kinase 1 expression in H9c2 cells using anti-Pyruvate dehydrogenase kinase 1 antibody 1:2,000. Green, isotype control; red, Pyruvate dehydrogenase kinase 1.

Western blotting analysis using anti-Pyruvate dehydrogenase kinase 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Pyruvate dehydrogenase kinsae 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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