Aliases:	Protein Phosphatase 2 Regulatory Subunit B'Epsilon; B56epsilon; B56E; Serine/Threonine-Protein Phosphatase 2A 56 KDa Regulatory Subunit Epsilon Isoform; Serine/Threonine Protein Phosphatase 2A, 56 KDa Regulatory Subunit, Epsilon; Protein Phosphatase 2, Regulatory Subunit B (B56), Epsilon Isoform; Protein Phosphatase 2 Regulatory Subunit B', Epsilon; PP2A B Subunit Isoform PR61-Epsilon; PP2A B Subunit Isoform B56-Epsilon; PP2A B Subunit Isoform B'-Epsilon; PP2A B Subunit Isoform R5-Epsilon; PP2A, B Subunit, PR61 Epsilon; PP2A, B Subunit, B56 Epsilon; PP2A, B Subunit, B' Epsilon; PP2A, B Subunit, R5 Epsilon
Background:	UniProt Entry: Q16537;NCBI Gene Entry: 5529
Application Information	Molecular Weight: Predicted, 55 kDa, observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1215 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PPP2R5E
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of PPP2R5E expression in HepG2 cells using anti-PPP2R5E antibody 1:2,000. Green, isotype control; red, PPP2R5E.

Immunocytochemical staining of HepG2 cells with anti-PPP2R5E antibody 1:1,000. Nuclei were stained blue with DAPI; PPP2R5E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-PPP2R5E antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively

Western blotting analysis using anti-PPP2R5E antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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