Aliases:	PRKAA1; Protein Kinase AMP-Activated Catalytic Subunit Alpha 1; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Protein Kinase, AMP-Activated, Alpha 1 Catalytic Subunit; Hydroxymethylglutaryl-CoA Reductase Kinase; Acetyl-CoA Carboxylase Kinase; Tau-Protein Kinase PRKAA1; AMPK Subunit Alpha-1; AMPK, Alpha, 1; ACACA Kinase; HMGCR Kinase; EC 2.7.11.1; AMPKa1; 5'-AMP-Activated Protein Kinase, Catalytic Alpha-1 Chain; AMP-Activated Protein Kinase, Catalytic, Alpha-1; AMP -Activate Kinase Alpha 1 Subunit; AMPK Alpha 1; EC 2.7.11.27; EC 2.7.11.31; EC 2.7.11.26; EC 2.7.11; AMPKA1; AMPK1; AMPK
Background:	UniProt Entry: Q13131;NCBI Gene Entry: 5562
Application Information	Molecular Weight: Predicted, 64 kDa, observed, 64 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2660 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human AMPK alpha 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-AMPK alpha 1 (S496) expression in HeLa cells using Phospho-AMPK alpha 1 (S496) antibody 1:2,000. Green, isotype control; red, Phospho-AMPK alpha 1 (S496).

Immunocytochemical staining of HeLa cells with Phospho-AMPK alpha 1 (S496) antibody 1:1,000. Nuclei were stained blue with DAPI; Phospho-AMPK alpha 1 (S496) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-AMPK alpha 1 (S496) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-AMPK alpha 1 (S496) antibody 1:5000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20000 respectively.

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