Aliases:	PRKCD; Protein Kinase C Delta; Tyrosine-Protein Kinase PRKCD; Protein Kinase C Delta Type; EC 2.7.11.13; NPKC-Delta; PKCD; Protein Kinase C Delta VIII; Protein Kinase C, Delta; EC 2.7.10.2; EC 2.7.11; ALPS3; CVID9; MAY1; PRKCQ; Protein Kinase C Theta; Protein Kinase C Theta Type; EC 2.7.11.13; NPKC-Theta; PRKCT; Protein Kinase C, Theta; EC 2.7.11
Background:	UniProt Entry: Q05655/ Q04759;NCBI Gene Entry: 5580/5588
Application Information	Molecular Weight: Predicted, 78,82 kDa; observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4585 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PKC delta + PKC theta
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-PKC delta + PKC theta antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using FeQTM ECL Substrate Kit .

Western blotting analysis using anti-PKC delta + PKC theta antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using FeQTM ECL Substrate Kit .

Flow cytometric analysis of PKC delta + PKC theta expression in HT-1080 cells using PKC delta + PKC theta antibody 1:2,000. Green, isotype control; red, PKC delta + PKC theta.

Immunocytochemical staining of HT-1080 cells with PKC delta + PKC theta antibody 1:1,000. Nuclei were stained blue with DAPI; PKC delta + PKC theta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

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