Aliases:	PRKDC; Protein Kinase, DNA-Activated, Catalytic Subunit; DNPK1; P460; DNA-Dependent Protein Kinase Catalytic Subunit; DNA-PKcs; DNA-PKC; DNAPK; XRCC7; HYRC1; P350; HYRC; Protein Kinase, DNA-Activated, Catalytic Polypeptide; DNA-PK Catalytic Subunit; EC 2.7.11.1; DNAPKc; Hyper-Radiosensitivity Of Murine Scid Mutation, Complementing 1; DNA-PKCS; DNAPKC; IMD26
Background:	UniProt Entry: P78527;NCBI Gene Entry: 5591
Application Information	Molecular Weight: Predicted, 469 kDa, observed, 469 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2075 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-DNA PKcs (S2056)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-DNA PKcs (S2056) expression in HT-1080 cells using Phospho-DNA PKcs (S2056) antibody 1:2,000. Green, isotype control; red, Phospho-DNA PKcs (S2056).

Immunocytochemical staining of HT-1080 cells with Phospho-DNA PKcs (S2056) antibody 1:1,000. Nuclei were stained blue with DAPI; Phospho-DNA PKcs (S2056) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-DNA PKcs (S2056) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-DNA PKcs (S2056) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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