Aliases:	Pre-MRNA Processing Factor 4; PRP4; U4/U6 Small Nuclear Ribonucleoprotein Prp4; SNRNP60; HPRP4P; Prp4p; HPRP4; PRP4/STK/WD Splicing Factor; U4/U6 SnRNP 60 KDa Protein; WD Splicing Factor Prp4; PRP4 Homolog; PRP4 Pre-MRNA Processing Factor 4 Homolog (Yeast); PRP4 Pre-MRNA Processing Factor 4 Homolog; HPrp4; RP70
Background:	UniProt Entry: O43172;NCBI Gene Entry: 9128
Application Information	Molecular Weight: Predicted, 58 kDa, observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB975 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PRPF4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of PRPF4 expression in HepG2 cells using anti-PRPF4 antibody 1:2,000. Green, isotype control; red, PRPF4.

Immunocytochemical staining of HepG2 cells with anti-PRPF4 antibody 1:1,000. Nuclei were stained blue with DAPI; PRPF4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-PRPF4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-PRPF4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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