Aliases:	PC4 And SRSF1 Interacting Protein 1; LEDGF; DFS70; Lens Epithelium-Derived Growth Factor; PSIP2; P52; P75; PC4 And SFRS1 Interacting Protein 1; Dense Fine Speckles 70 KDa Protein; PC4 And SFRS1-Interacting Protein; CLL-Associated Antigen KW-7; PC4 And SFRS1 Interacting Protein 2; Transcriptional Coactivator P52/P75; Transcriptional Coactivator P75/P52; DFS 70; PAIP
Background:	UniProt Entry: O75475;NCBI Gene Entry: 11168
Application Information	Molecular Weight: Predicted, 60 kDa, observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB840 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PSIP1 / LEDGF
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of PSIP1 expression in HeLa cells using PSIP1 antibody 1:2,000. Green, isotype control; red, PSIP1.

Immunocytochemical staining of Hela cells with anti-PSIP1 antibody 1:1,000. Nuclei were stained blue with DAPI; PSIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-PSIP1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-PSIP1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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