Aliases:	RACK1; Receptor For Activated C Kinase 1; GNB2L1; H12.3; Guanine Nucleotide Binding Protein (G Protein), Beta Polypeptide 2-Like 1; Guanine Nucleotide-Binding Protein Subunit Beta-Like Protein 12.3; Guanine Nucleotide-Binding Protein Subunit Beta-2-Like 1; Cell Proliferation-Inducing Gene 21 Protein ; Receptor Of Activated Protein C Kinase 1; Small Ribosomal Subunit Protein RACK1; Human Lung Cancer Oncogene 7 Protein ; Gnb2-Rs1; HLC-7; Protein Homologous To Chicken B Complex Protein, Guanine Nucleotide Binding; Guanine Nucleotide Binding Protein Beta Polypeptide 2-Like 1; Receptor Of Activated Protein Kinase C 1; Receptor For Activated C Kinase; Proliferation-Inducing Gene 21; Lung Cancer Oncogene 7; GNB2-RS1; PIG21
Background:	UniProt Entry: PKD-10452;NCBI Gene Entry: 10399
Application Information	Molecular Weight: Predicted, 35 kDa, observed, 35 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2355 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RACK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of RACK1 expression in HepG2 cells using RACK1 antibody 1:2,000. Green, isotype control; red, RACK1.

Immunocytochemical staining of HepG2 cells with RACK1 antibody 1:1,000. Nuclei were stained blue with DAPI; RACK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-RACK1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-RACK1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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