Aliases:	RAD21; RAD21 Cohesin Complex Component; HHR21; SCC1; KIAA0078; Double-Strand-Break Repair Protein Rad21 Homolog; Sister Chromatid Cohesion 1; Nuclear Matrix Protein 1; SCC1 Homolog; Kleisin; NXP-1; HR21; NXP1; Protein Involved In DNA Double-Strand Break Repair; RAD21 (S. Pombe) Homolog; RAD21 Homolog (S. Pombe); RAD21 Homolog; HRAD21; CDLS4; MCD1; MGS
Background:	UniProt Entry: O60216;NCBI Gene Entry: 5885
Application Information	Molecular Weight: Predicted, 72 kDa, observed, 130 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1390 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human RAD21
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of RAD21 expression in HepG2 cells using anti-RAD21 antibody 1:2,000. Green, isotype control; red, RAD21.

Immunocytochemical staining of HepG2 cells with anti-RAD21 antibody 1:1,000. Nuclei were stained blue with DAPI; RAD21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-RAD21 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-RAD21 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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