Aliases:	RAF1; Raf-1 Proto-Oncogene, Serine/Threonine Kinase; Raf-1; CRAF; RAF Proto-Oncogene Serine/Threonine-Protein Kinase; V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1; C-Raf Proto-Oncogene, Serine/Threonine Kinase; Proto-Oncogene C-RAF; EC 2.7.11.1; C-Raf; V-Raf-1 Murine Leukemia Viral Oncogene-Like Protein 1; Raf Proto-Oncogene Serine/Threonine Protein Kinase; Oncogene RAF1; EC 2.7.11; CMD1NN; C-RAF; RAF-1; CRaf; NS5; RAF
Background:	UniProt Entry: P04049;NCBI Gene Entry: 5894
Application Information	Molecular Weight: Predicted, 73 kDa, observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2650 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-Raf1 (S621)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Phospho-Raf1 (S621) expression in HeLa cells using Phospho-Raf1 (S621) antibody 1:2,000. Green, isotype control; red, Phospho-Raf1 (S621).

Immunocytochemical staining of HeLa cells with Phospho-Raf1 (S621) antibody 1:1,000. Nuclei were stained blue with DAPI; Phospho-Raf1 (S621) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-Raf1 (S621) antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Phospho-Raf1 (S621) antibody 1:5000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20000 respectively.

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