Aliases:	RELA; RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells; NFKB3; P65; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; Transcription Factor P65; NF-Kappa-B Transcription Factor P65; NF-Kappa-B; P65delta3; AIF3BL3; CMCU
Background:	UniProt Entry: Q04206;NCBI Gene Entry: 5970
Application Information	Molecular Weight: Predicted, 60 kDa, observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1490 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NF-κB p65
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of NF-kB p65 expression in HepG2 cells using NF-kB p65 antibody 1:2,000. Green, isotype control; red, NF-kB p65.

Immunocytochemical staining of HepG2 cells with NF-kB p65 antibody 1:1,000. Nuclei were stained blue with DAPI; NF-kB p65 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-NF-kB p65 antibody 1:10,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-NF-kB p65 antibody 1:10,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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