Aliases:	SHH; Sonic Hedgehog Signaling Molecule; MCOPCB5; SMMCI; TPTPS; HHG1; TPT; Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains; Sonic Hedgehog Protein; ShhNC; HLP3; HPE3; Sonic Hedgehog (Drosophila) Homolog; Sonic Hedgehog Homolog; EC 3.1.-.-; HHG-1
Background:	UniProt Entry: Q15465;NCBI Gene Entry: 6469
Application Information	Molecular Weight: Predicted, 50 kDa, observed, 51 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1925 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Sonic Hedgehog
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Sonic Hedgehog expression in HepG2 cells using anti-Sonic Hedgehog antibody 1:2,000. Green, isotype control; red, Sonic Hedgehog.

Immunocytochemical staining of HepG2 cells with anti-Sonic Hedgehog antibody 1:1,000. Nuclei were stained blue with DAPI; Sonic Hedgehog was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-sonic hedgehog antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-sonic hedgehog antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit . SHH, sonic hedgehog signaling molecule.

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