Aliases:	SMAD1; SMAD Family Member 1; MADR1; JV4-1; MADH1; Mothers Against Decapentaplegic Homolog 1; Mothers Against DPP Homolog 1; Mad-Related Protein 1; BSP-1; BSP1; MAD, Mothers Against Decapentaplegic Homolog 1 (Drosophila); Transforming Growth Factor-Beta Signaling Protein 1; Transforming Growth Factor-Beta-Signaling Protein 1; SMAD, Mothers Against DPP Homolog 1 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 1; SMAD, Mothers Against DPP Homolog 1; TGF-Beta Signaling Protein 1; MAD Homolog 1; SMAD 1; HSMAD1; Smad1; JV41
Background:	UniProt Entry: Q15797;NCBI Gene Entry: 4086
Application Information	Molecular Weight: Predicted, 52 kDa, observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2100 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM)
Immunogen	A synthesized peptide derived from human Smad1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of SMAD1 expression in HAP-1 cells using anti-SMAD1 antibody 1:2,000. Green, isotype control; red, SMAD1.

Western blotting analysis using anti-SMAD1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-SMAD1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .

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