Aliases:	UQCRFS1; Ubiquinol-Cytochrome C Reductase, Rieske Iron-Sulfur Polypeptide 1; RISP; Cytochrome B-C1 Complex Subunit 5; UQCR5; RIS1; RIP1; Cytochrome B-C1 Complex Subunit Rieske, Mitochondrial; Ubiquinol-Cytochrome C Reductase Iron-Sulfur Subunit; Rieske Iron-Sulfur Protein; Rieske Protein UQCRFS1; Complex III Subunit 5; Epididymis Secretory Sperm Binding Protein; EC 1.10.2.2; EC 7.1.1.8; MC3DN10
Background:	UniProt Entry: P47985;NCBI Gene Entry: 7386
Application Information	Molecular Weight: Predicted, 30 kDa, observed, 23 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2460 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human UQCRFS1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of UQCRFS1 expression in HepG2 cells using anti-UQCRFS1 antibody 1:2,000. Green, isotype control; red, UQCRFS1.

Immunocytochemical staining of HepG2 cells with anti-UQCRFS1 antibody 1:1,000. Nuclei were stained blue with DAPI; UQCRFS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-UQCRFS1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-UQCRFS1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .