Aliases:	USP5; Ubiquitin Specific Peptidase 5; Isopeptidase T; IsoT; Ubiquitin-Specific-Processing Protease 5; Ubiquitin Carboxyl-Terminal Hydrolase 5; Deubiquitinating Enzyme 5; Ubiquitin Thioesterase 5; Ubiquitin-Specific Protease-5 (Ubiquitin Isopeptidase T); Ubiquitin Specific Peptidase 5 (Isopeptidase T); Ubiquitin Specific Protease 5 (Isopeptidase T); Testicular Tissue Protein Li 218; Ubiquitin Thiolesterase 5; Ubiquitin Isopeptidase T; EC 3.4.19.12; EC 3.1.2.15; ISOT
Background:	UniProt Entry: P45974;NCBI Gene Entry: 8078
Application Information	Molecular Weight: Predicted, 96 kDa, observed, 100 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2480 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human USP5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Ubiquitin specific peptidase 5 expression in HepG2 cells using anti-Ubiquitin specific peptidase 5 antibody 1:2,000. Green, isotype control; red, Ubiquitin specific peptidase 5.

Immunocytochemical staining of HepG2 cells with anti-Ubiquitin specific peptidase 5 antibody 1:1,000. Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Ubiquitin specific peptidase 5 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Ubiquitin specific peptidase 5 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .