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One Step ELISA Kit For Human Tumor Necrosis Factor Alpha (TNFa)
One Step ELISA Kit For Human Tumor Necrosis Factor Alpha (TNFa)
DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
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  • NO.:One-EK003H
    Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
    Species:Human
    Assay type:One step sandwich elisa
    Validity:Two Years
    Assay Time:1.5 hours
    Assay range:3.13 pg/mL–200 pg/mL
    Sensitivity:1.77 pg/mL
    NCBI_Gene:7124
    UniProtKB:P01375
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Details

SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 10μL; DMEM cell culture supernatant: 33μL
SENSITIVITY 1.77 pg/mL
RANGE 3.13 pg/mL – 200 pg/mL
ASSAY TIME 1.5 h
RECOVERY 100% – 122%
AVERAGE RECOVERY 1.08
INTRA PRECISION 4.2% – 5.4%
INTER-PRECISION 5.5% – 6.2%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody
Human TNF-α freeze-dried standard
TNF-α detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human TNF-α has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for TNF-α (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then TNF-α present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of TNF-α bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader.


 

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