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One Step ELISA Kit For Mouse Interleukin 6 (IL6)
One Step ELISA Kit For Mouse Interleukin 6 (IL6)
MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
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  • NO.:One-EK001M
    Species:Mouse
    Assay range:7.81 pg/mL – 500 pg/mL
    Sensitivity:4.92 pg/mL
    Sample Volume:Serum, plasma:10 μL
    Assay type:One step sandwich elisa
    Validity:Two Years
    Assay Time:1.5 hours
    NCBI_Gene:3569
    UniProtKB:P05231
  • Goods click count:551
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Details

SAMPLE TYPE
Cell culture supernates, serum and plasma
SAMPLE VOLUME Serum or plasma: 10μL;  cell culture supernatant: 50μL
SENSITIVITY 4.92 pg/mL
RANGE 7.81 pg/mL – 500 pg/mL
ASSAY TIME 1.5 h
RECOVERY 89% – 112%
AVERAGE RECOVERY 1.02
INTRA PRECISION 2.3% – 3.0%
INTER-PRECISION 2.1% – 5.3%
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Mouse IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

 

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