Product overview

Human Anti-Elastase IgG (Anti-ELA-IgG) ELISA Kit (Catalog No. SLD4184Hu) is an enzyme-linked immunosorbent assay kit for detection of the target in In serum, plasma, culture media or any biological fluid.

Assay principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Anti-ELA-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for ELA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Anti-ELA-IgG and HRP conjugated ELA antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Anti-ELA-IgG. You can calculate the concentration of Anti-ELA-IgG in the samples by comparing the OD of the samples to the standard curve.

Specifications

• Assay range: 10ng/ml-400ng/ml
• Sensitivity: 1ng/ml
• Standard: 450ng/ml
• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

Kit components

• User manual
• Closure plate membrane
• Sealed bags
• Pre-coated microelisa stripplate
• Standard (450ng/ml)
• Standard diluent
• HRP-conjugate reagent
• Sample diluent
• Chromogen Solution A
• Chromogen Solution B
• Stop Solution

Sample type

In serum, plasma, culture media or any biological fluid.

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)