Animal transmembrane proteins undertake various biological functions and play an important role in the occurrence and development of diseases. The preparation of membrane protein samples needs to fully consider the downstream applications such as gel analysis and mass spectrometry analysis, so the preparation of membrane protein samples has become an insurmountable challenge. The membrane protein extraction kit is a fast, efficient and high-yield membrane protein extraction kit. This kit provides a complete set of reagents suitable for extracting total membrane proteins from animal cells and animal tissues. The extraction process is simple and convenient and can be completed within 1 hour. The extracted membrane proteins not only have high purity and maintain natural activity, but also have minimal cross-contamination. The unique formula contained in this kit can effectively dissolve cell membrane components, including cytoplasmic membrane, nuclear membrane and various organelle membranes. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis. It does not contain SDS, TritonX-100, chaps and other components that may affect mass spectrometry experiments. It can basically meet any downstream proteomics-related experiments. Research. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid mass spectrum peak drift caused by AEBSF. Therefore, protein samples extracted with this product can be used for mass spectrometry (MS) detection and analysis, proteomics, etc. Related research. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: animal cells/tissues

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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