The nuclear protein extraction kit provides a complete set of reagents, suitable for extracting from various primary or passaged animal cells and various animal solid tissues, such as brain, spinal cord, neural nodes or fibers, fat, liver, digestive tract, kidney, heart Extract nuclear and cytoplasmic proteins from animal tissues such as muscles, blood vessels, connective tissues, etc. The extraction process is simple and convenient and can be completed within 1 hour. The prepared nuclear proteins and cytoplasmic proteins not only have high purity and maintain natural activity, but also have very little cross-contamination. This kit contains a unique formula that can effectively dissolve nuclear membrane components. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases and ensures the extraction of high-purity proteins. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, TritonX-100, chaps and other components that may affect the mass spectrometry experiment. The final protein sample is dialyzed or desalted. It will not contain the effects of detergents, high-concentration salts, etc., and can basically meet any downstream proteomics-related experimental research. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid mass spectrum peak drift caused by AEBSF. Therefore, protein samples extracted with this product can be used for mass spectrometry (MS) detection and analysis, proteomics, etc. Related research. The proteins extracted by this kit are active proteins with natural protein conformation.

 

Applicable samples: animal cells/tissues

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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