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Sunlong Medical™ Mouse IL-2 High Sensitivity ELISA Kit
Sunlong Medical™ Mouse IL-2 High Sensitivity ELISA Kit
IL2 elisa kit | interleukin 2 elisa kit | IL-2 | Il-2
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Regular members: $524.0
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  • NO.:HS-EL0113Mo
    Species:Mouse
    Assay range:0.23pg/mL-15pg/mL
    Sensitivity:0.05 pg/mL
    Sample Volume:20 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:321
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Cat NO.:
HS-EL0113Mo
Product:
Sunlong Medical™ Mouse IL-2 High Sensitivity ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
82% - 98%
Mean Spike Recovery:
0.92
CV of Intra plate:
4.5% - 4.9%
CV of Inter plate:
4.4% - 8.9%
NCBI_Gene:
16183
UniProtKB:
P04351

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-2 monoclonal antibody
Mouse IL-2 freeze-dried standard
IL-2 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-2 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-2 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
















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