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Sunlong Medical™ Mouse IL-1β High Sensitivity ELISA Kit
Sunlong Medical™ Mouse IL-1β High Sensitivity ELISA Kit
IL1B elisa kit | interleukin 1 beta elisa kit | IL-1B | Il-1b | IL-1beta
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Regular members: $524.0
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  • NO.:HS-EL0115Mo
    Species:Mouse
    Assay range:1.563pg/mL-100pg/mL
    Sensitivity:0.07 pg/mL
    Sample Volume:Serum | plasma;10 μL;Cell culture supernatant;100 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:358
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Cat NO.:
HS-EL0115Mo
Product:
Sunlong Medical™ Mouse IL-1β High Sensitivity ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
82% - 110%
Mean Spike Recovery:
0.99
CV of Intra plate:
2.8% - 3.6%
CV of Inter plate:
2.6%-3.2%
NCBI_Gene:
16176
UniProtKB:
P10749

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1β monoclonal antibody
Mouse IL-1β freeze-dried standard
IL-1β detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-1β antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1β present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
















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Em***22024-09-07
Ad***32024-08-28
Th***12024-08-22
Is***32024-08-19
Ta***12024-07-26
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