TEL: +86 571 56623320 EMAIL: [email protected]
Cat NO.:HS-EL0115Mo Product:Sunlong Medical™ Mouse IL-1β High Sensitivity ELISA Kit Storage4℃ (unopened) for two years Shipping Condition4℃ Sample Type:Serum | plasma | cell culture supernatant and other biological samples Spike Recovery Range:82% - 110% Mean Spike Recovery:0.99 CV of Intra plate:2.8% - 3.6% CV of Inter plate:2.6%-3.2% NCBI_Gene:16176 UniProtKB:P10749
PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1β monoclonal antibody
Mouse IL-1β freeze-dried standard
IL-1β detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing FilmASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-1β antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1β present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
11. Platycodonis Radix Alleviates LPS-Induced Lung Inflammation through Modulation of TRPA1 Channels
Username | Quantity | bought time |
Em*** | 2 | 2024-09-07 |
Ad*** | 3 | 2024-08-28 |
Th*** | 1 | 2024-08-22 |
Is*** | 3 | 2024-08-19 |
Ta*** | 1 | 2024-07-26 |
Scan Wechat Qrcode
Scan Whatsapp Qrcode