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Sunlong Medical™ Human IL-8 High Sensitivity ELISA Kit
Sunlong Medical™ Human IL-8 High Sensitivity ELISA Kit
CXCL8 elisa kit | C-X-C motif chemokine ligand 8 elisa kit | alveolar macrophage chemotactic factor I | beta endothelial cell-derived neutrophil activating peptide | beta-thromboglobulin-like protein | C-X-C motif chemokine 8 | chemokine C-X-C motif lig
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  • NO.:HS-EL0274Hu
    Species:Human
    Assay range:2.34pg/mL-150pg/mL
    Sensitivity:0.28 pg/mL
    Sample Volume:Serum | plasma;50 μL;Cell culture supernatant;100 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:355
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Cat NO.:
HS-EL0274Hu
Product:
Sunlong Medical™ Human IL-8 High Sensitivity ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
91% - 114%
Mean Spike Recovery:
1.02
CV of Intra plate:
4.3% - 5.0%
CV of Inter plate:
3.3% - 4.7%
NCBI_Gene:
3576
UniProtKB:
P10145

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-8 monoclonal antibody
Human IL-8 high sensitivity freeze-dried standard
IL-8 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-8 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-8 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-8 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
















 

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Ra***22024-09-09
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