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Sunlong Medical™ Human IL-6 High Sensitivity ELISA Kit
Sunlong Medical™ Human IL-6 High Sensitivity ELISA Kit
IL6 elisa kit | interleukin 6 elisa kit | B cell stimulatory factor-2 | B-cell differentiation factor | B-cell stimulatory factor 2 | BSF-2 | BSF2 | CDF | CTL differentiation factor | HGF | HSF | hybridoma growth factor | IFN-beta-2 | IFNB2 | IL-6 | inter
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  • NO.:HS-EL0277Hu
    Species:Human
    Assay range:0.31pg/mL-20pg/mL
    Sensitivity:0.02 pg/mL
    Sample Volume:20 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:400
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Cat NO.:
HS-EL0277Hu
Product:
Sunlong Medical™ Human IL-6 High Sensitivity ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
74% - 119%
Mean Spike Recovery:
0.94
CV of Intra plate:
4.7% - 5.6%
CV of Inter plate:
4.6% - 6.7%
NCBI_Gene:
3569
UniProtKB:
P05231

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Human IL-6 freeze-dried standard
IL-6 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-6 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-6 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
















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Ja***12024-09-07
Ch***22024-09-06
Ad***32024-06-07
Zo***22024-06-03
Ka***22024-05-27
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