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Cat NO.:HS-EL0261Hu Product:Sunlong Medical™ Human IL-10 High Sensitivity ELISA Kit Storage4℃ (unopened) for two years Shipping Condition4℃ Sample Type:Serum | plasma | cell culture supernatant and other biological samples Spike Recovery Range:88% - 100% Mean Spike Recovery:0.92 CV of Intra plate:3.5% - 4.1% CV of Inter plate:4.0% - 8.8% NCBI_Gene:3586 UniProtKB:P22301
PLATFORM ELISA PLATE Detachable 96-well plate SIZE 96T/48T STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. DELIVERY 4℃ blue ice transportation COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody
Human IL-10 freeze-dried standard
IL-10 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing FilmASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-10 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-10 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
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