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Sunlong Medical™ Rat IL-6 High Sensitivity ELISA Kit
Sunlong Medical™ Rat IL-6 High Sensitivity ELISA Kit
IL6 elisa kit | interleukin 6 elisa kit | Ifnb2 | IL-6 | ILg6 | Interleukin 6 interferon beta 2 | Interleukin 6 interferon | beta 2 | interleukin-6
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  • NO.:HS-EL0033Ra
    Species:Rat
    Assay range:7.81pg/mL-500pg/mL
    Sensitivity:1.8 pg/mL
    Sample Volume:20 μL
    Assay type:Sandwich Method
    Validity:Two Years
  • Goods click count:358
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Cat NO.:
HS-EL0033Ra
Product:
Sunlong Medical™ Rat IL-6 High Sensitivity ELISA Kit
Storage
4℃ (unopened) for two years
Shipping Condition
4℃
Sample Type:
Serum | plasma | cell culture supernatant and other biological samples
Spike Recovery Range:
95% - 106%
Mean Spike Recovery:
1.02
CV of Intra plate:
6.4% - 9.1%
CV of Inter plate:
6.1% - 9.6%
NCBI_Gene:
24498
UniProtKB:
P20607

 

PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Rat IL-6 freeze-dried standard
IL-6 detect Antibody
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-6 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-6 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).
















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Ta***22024-09-06
Ta***12024-08-29
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