Description

The One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing  detection  throughput  and   reducing  the  risk  of  contamination.  This   Kit  introduced dUTP/UDG system.  Heat-labile  UDG  can  rapidly  degrade the  pollutants  containing  U  at  room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly  without  affecting  the   efficiency  and  sensitivity  of   RT-qPCR.   Integrating  the  superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0. 1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5 × One-step U+  Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan® . This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.
Components

 

Component	SLPCR228-1 200 rxn, 20 μl/rxn	SLPCR228-2 1000 rxn, 20 μl/rxn
5 × One-step U+  Mixa	800 µl	1 ml × 4
One-step U+  Enzyme Mix V3b	200 µl	1 ml
RNase-free ddH2O	1 ml	15 ml

 

a.    Contains dNTP/dUTP Mix, Mg2+ .

b.    Contains  Reverse  Transcriptase,  RNase  inhibitor,  Heat-labile  UDG  and  hot-start  Taq  DNA Polymerase.

Storage

This reagent should be kept at -20°C.

Cartpieces

• 
• 1

  1Delete

• 
• 7

  7Delete

• 
• S

  SDelete

• 
• O

  ODelete

• 
• +

  +Delete

• 
• 4

  4Delete

• 
• 2

  2Delete

• Delete

• 
• 1

  1Delete

• 
• 2

  2Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• O

  ODelete

• Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• 
• w

  wDelete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• O

  ODelete

• 
• 5

  5Delete

• 
• P

  PDelete

Totalgoods,subtotals:￥Checkout