Introduction

FastKing One Step  RT-PCR  Kit allows  both  reverse transcription and gene amplification to take place in a single tube, which avoids cross contamination between samples and improves the sensitivity of detection. The 25×RT-PCR Enzyme Mix contains King reverse transcriptase, which is a high efficient reverse transcriptase expressed by engineering bacteria; a further-modified hot start Taq DNA polymerase, which provides high efficiency and accuracy for the amplify reaction; and RNase inhibitor. With a special modified hydrophobic motif, King RTase gets a significant affinity for RNA and facilitates transcription through of RNA templates, especially for templates with high GC content or complex secondary structures. The 2×FastKing One Step RT-PCR MasterMix contains appropriate ion concentration, dNTPs and PCR enhancer. It could stabilize both RTase and polymerases and keep their efficiency within the whole reaction process.

 

  Materials required but not supplied

1. RNA templates

2. gene-specific PCR primers

 

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