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Rabbit Anti-phospho-MAP4 (Ser1046)/AF594 Conjugated antibody
Rabbit Anti-phospho-MAP4 (Ser1046)/AF594 Conjugated antibody
MAP4(phospho Ser1046); DKFZp779A1753; MAP-4; MAP4; MAP4_MOUSE; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730.
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Product Name Anti-phospho-MAP4(Ser1046)/AF594
Chinese Name AF594标记的磷酸化微管相关蛋白4抗体
Alias MAP4(phospho Ser1046); DKFZp779A1753; MAP-4; MAP4; MAP4_MOUSE; MGC8617; Microtubule associated protein 4; Microtubule-associated protein 4; OTTHUMP00000210723; OTTHUMP00000210725; OTTHUMP00000210727; OTTHUMP00000210730.  
Product Type Phosphorylated anti 
Research Area Cell biology  Signal transduction  Cyclin  Cytoskeleton  
Immunogen Species Rabbit
Clonality Polyclonal
React Species
Applications ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Molecular weight 117kDa
Form Lyophilized or Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthesised phosphopeptide derived from mouse MAP4 around the phosphorylation site of Ser1046
Lsotype IgG
Purification affinity purified by Protein A
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Product Detail background:
The MAP4 (Microtubule associated protein 4)is a non neuronal microtubule associated protein. It promotes microtubule assembly.The phosphorylation of the Pro rich region in the C-terminus negatively regulates MAP4 activity to promote microtubule assembly.

Function:
Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

Subunit:
Interacts with SEPT2; this interaction impedes tubulin-binding (By similarity).

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Testis, striated and cardiac muscle.

Post-translational modifications:
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-760 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 4 is phosphorylated on Ser-333 and Ser-334 (By similarity).

Similarity:
Contains 4 Tau/MAP repeats.

Database links:
UniProtKB/Swiss-Prot: P27546.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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