Product Name	NSBP1
Chinese Name	核小体Binding protein1抗体
Alias	Nucleosome-binding protein 1; Nucleosome-binding protein 45; NBP-45; GARP45 protein; HMGN5_HUMAN.
Research Area	immunology transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NSBP1: 201-282/282
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	NSBP1 binds specifically to nuclear core particles and acts as a transcriptional activator. [Subcellular Location] Nucleus. [Tissue Specificity] Widely expressed with highest levels in submaxillary gland, thymus, kidney and liver and lowest levels in brain, lung, pancreas and eye. [Developmental Stage] Highest levels are found in 7-day-old embryos. Levels in the 7-day-old embryo are 4-fold higher than in the adult and almost 10-fold higher than in later embryonic stages. [SIMILARITY] Belongs to the HMGN family. Function: Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction (By similarity). Subcellular Location: Nucleus. Note=Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin (By similarity). Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the HMGN family. SWISS: P82970 Gene ID: 79366 Database links: Entrez Gene: 79366 Human Omim: 300385 Human SwissProt: P82970 Human Unigene: 282204 Human NSBP-1主要用于前列腺疾病方面的研究。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSBP1 ) Polyclonal Antibody, Unconjugated (SL0700R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 1M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NSBP1 Polyclonal Antibody, Unconjugated(SL0700R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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