Product Name	Glutathione Reductase
Chinese Name	谷胱甘肽还原酶抗体
Alias	glutathione reductase; GLUR; Glutathione reductase mitochondrial; GR; Gr1; GRase; GRD 1; GRD1; GSR; MGC78522; GSHR_HUMAN.
literatures	Specific References (2) | SL0837R has been referenced in 2 publications. [IF=3.69] Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873 WB ; Human. PubMed:33485970 [IF=2.134] Khan et al. Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction. (2013) Biomed.Res.In. 2013:719858 WB ; Human. PubMed:23533997
Research Area	immunology Kinases and Phosphatases Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,Mouse,Rat(predicted:Dog,Pig,Cow)
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	57kDa
Cellular localization	Secretory protein Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Glutathione Reductase: 421-522/522
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Human,Mouse,Rat(predicted:Dog,Pig,Cow)1M TBS(pH7.4) with 1% BSA, Human,Mouse,Rat(predicted:Dog,Pig,Cow)3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. Function: Maintains high levels of reduced glutathione in the cytosol. Subunit: Homodimer; disulfide-linked. Subcellular Location: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. SWISS: P00390 Gene ID: 2936 Database links: Entrez Gene: 2936 Human Entrez Gene: 14782 Mouse Entrez Gene: 116686 Rat Omim: 138300 Human SwissProt: P00390 Human SwissProt: P47791 Mouse SwissProt: P70619 Rat Unigene: 271510 Human Unigene: 283573 Mouse Unigene: 19721 Rat 胱甘肽还原酶为一种黄素酶，可催化氧化型谷胱甘肽转变成还原型谷胱甘肽(GSH)，从而提高细胞液中GSH的含量。 GSH具有抵抗自由基氧化对机体损伤的作用，GSH的重要生理功能在于能还原体内生成的过氧化氢(H2O2)。GSH的储量与创伤、Tumour等的预后，以及人体衰老有关。
Product Picture	Sample: A549(Human) Cell Lysate at 30 ug SP2/0(Mouse) Cell Lysate at 30 ug Primary: Anti-Glutathione Reductase (SL0837R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (SL0837R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human Pulp); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (SL0837R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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