Product Name	Caspase-1 p20
Chinese Name	天冬氨酸-胱氨酸特异性蛋白酶家族抗体
Alias	caspase-1 isoform alpha p20 subunit; CASP1; ICE; IL1BC; Caspase-1 subunit p20; CASP 1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; CASP1_HUMAN; CASP1_MOUSE.
literatures	Specific References (15) | SL10442R has been referenced in 15 publications. [IF=11.799] Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134 IF,IHC ; mouse. PubMed:34600334 [IF=9.381] Xianglin Pan. et al. Pectic polysaccharide from Smilax china L. ameliorated ulcerative colitis by inhibiting the galectin-3/NLRP3 inflammasome pathway. Carbohyd Polym. 2022 Feb;277:118864 WB ; Mouse. PubMed:34893269 [IF=7.561] You Zhou. et al. Overexpression of lncRNA TUG1 Alleviates NLRP3 Inflammasome-Mediated Cardiomyocyte Pyroptosis Through Targeting the miR-186-5p/XIAP Axis in Coronary Microembolization-Induced Myocardial Damage. Front Immunol. 2021; 12: 637598 WB ; Rat. PubMed:34163467 [IF=6.633] Shuai Jiang. et al. Cardiac-specific overexpression of Claudin-5 exerts protection against myocardial ischemia and reperfusion injury. BBA-MOL BASIS DIS. 2022 Sep;:166535 WB ; Mouse. PubMed:36058416 [IF=6.304] Hanwen Li et al. P2Y 14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death Dis.2020 May 26;11(5):394. WB ; Rat. PubMed:32457291 [IF=5.895] Xu Yang. et al. T-2 Toxin Caused Mice Testicular Inflammation Injury via ROS-Mediated NLRP3 Inflammasome Activation. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX WB ; Mouse. PubMed:36260425 [IF=5.714] Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-κB signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192 WB ; Human. PubMed:37068341 [IF=4.966] Hongli Guo. et al. Transcriptional enhancement of GBP-5 by BATF aggravates sepsis-associated liver injury via NLRP3 inflammasome activation. Faseb J. 2021 Jun;35(6):e21672 FC ; Mouse. PubMed:34042221 [IF=4.268] Xiping Yang. et al. The piperine derivative HJ105 inhibits Aβ1-42-induced neuroinflammation and oxidative damage via the Keap1-Nrf2-TXNIP axis. Phytomedicine. 2021 Apr;:153571 WB ; Rat. PubMed:33994056 [IF=3.921] Meiyun Cai. et al. Kaemperfol alleviates pyroptosis and microglia-mediated neuroinflammation in Parkinson's disease via inhibiting p38MAPK/NF-κB signaling pathway. Neurochem Int. 2021 Nov;:105221 WB ; Rat. PubMed:34780806 [IF=3.59] Jie Ma. et al. The Effect of MaxiK Channel on Regulating the Activation of NLRP3 Inflammasome in Rats of Blast-induced Traumatic Brain Injury. Neuroscience. 2022 Feb;482:132 WB ; Rat. PubMed:34923036 [IF=3.361] Wang Z et al. Secoisolariciresinol diglucoside suppresses Dextran sulfate sodium salt-induced colitis through inhibiting NLRP1 inflammasome. Int Immunopharmacol. 2020 Jan;78:105931. WB&ICF ; Mouse. PubMed:31812068 [IF=3.246] Guo-Ping Wang. et al. To Analyze the Mechanism of SalB Regulating SIRT1 to Inhibit NLRP3 and Its Ameliorative Effect on Tubulogastric Junction Tumor Lesions Complicated with Myocardial Injury. BIOMED RES INT. 2022 Oct 14;2022:6560693 WB ; Rat. PubMed:36277894 [IF=1.662] Jia-Jia Xu. et al. Anti-inflammatory effect of a polysaccharide fraction from Craterellus cornucopioides in LPS-stimulated macrophages. 2021 Jun 30 WB ; Mouse. PubMed:34189750 [IF=0.439] Chen JW et al. Zhihuang Tongfeng decoction ameliorates gouty arthritis via inhibition of NLRP3 inflammasome in rats. Tropical Journal of Pharmaceutical Research December 2019; 18 (12): 2577-2582. WB ; Rat. PubMed:doi:10.4314/tjpr.v18i12.17
Research Area	Tumour Cell biology Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	20/46kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse Caspase-1 p20: 181-280/402
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 1M PBS, pH 7.4
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]. Function: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Post-translational modifications : The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P29452 Gene ID: 12362 Database links: Entrez Gene: 12362 Mouse Entrez Gene: 25166 Rat SwissProt: P29466 Human SwissProt: P29452 Mouse SwissProt: P43527 Rat Unigene: 1051 Mouse Unigene: 37508 Rat
Product Picture	Sample:large intestine (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(SL10442R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD Sample:lung (rat) Lysate at 40 ug Primary: Anti-Caspase-1 p20(SL10442R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20/46kD Observed band size: 46kD Tissue/cell: Mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 1M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-Caspase-1 p20 Polyclonal Antibody, Unconjugated(SL10442R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (SL10442R), Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC<,Dilution: 1μg /test. Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-Caspase-1 p20 antibody (SL10442R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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