Product Name	DGKI
Chinese Name	甘油二酯激酶ι/DGK-ι抗体
Alias	DAG kinase iota; DGK iota; DGKiota; DGKI_HUMAN; Diacylglycerol kinase iota; Diglyceride kinase iota.
Research Area	Tumour Cell biology Signal transduction Kinases and Phosphatases The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human
Applications	WB=1:500-2000 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	117kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DGKI: 101-200/1065
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Human1M TBS(pH7.4) with 1% BSA, Human3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008] Function: This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. Subcellular Location: Cytoplasm. Nucleus. SWISS: O75912 Gene ID: 9162 Database links: Entrez Gene: 9162 Human Entrez Gene: 688705 Rat Omim: 604072 Human SwissProt: O75912 Human
Product Picture	Sample:Hela (Human)Cell Lysate at 40 ug Primary: Anti-DGKI(SL14299R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 117kD Observed band size: 117kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGKI) Polyclonal Antibody, Unconjugated (SL14299R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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